Glass slide-based agarose gel electrophoresis for determining the efficiency of RNA amplification and cyanine dye incorporation during RNA labeling.
نویسندگان
چکیده
منابع مشابه
Isolation of high-quality RNA from a wide range of woody plants
Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...
متن کاملCounter-selection facilitated plasmid construction by homologous recombination in Saccharomyces cerevisiae.
Vol. 35, No. 4 (2003) BioTechniques 693 points are distributed along lines that represent the expected differences in signal intensity from each channel. Without a systematic dye effect or after proper normalization, we would see three sets of data points along three equally spaced lines parallel with slope one. On the other hand, the direct comparison of measurements for each dye on different ...
متن کاملApplications of competitor RNA in diagnostic reverse transcription-PCR.
Detection of RNA viruses by reverse transcription (RT)-PCR has proven to be a useful approach for the diagnosis of infections caused by many viral pathogens. However, adequate controls are required for each step of the RT-PCR protocol to ensure the accuracies of diagnostic test results. Heterologous competitor RNA can be used as a control for a number of different aspects of diagnostic RT-PCR. ...
متن کاملDetermining a significant change in protein expression with DeCyder during a pair-wise comparison using two-dimensional difference gel electrophoresis.
Two-dimensional difference gel electrophoresis (DIGE) is a tool for measuring changes in protein expression between samples involving pre-electrophoretic labeling with cyanine dyes. Here we assess a common method to analyze DIGE data using the DeCyder software system. Experimental error was studied by a series of same sample comparisons. Aliquots of sample were labeled with N-hydroxyl succinimi...
متن کاملLinear RNA Amplification and Microarray Hybridizations Total RNA was isolated from a human breast tumor, a human lung squamous cell carcinoma, and from two cultured breast tumor epithelial cell lines
DNA microarrays are a powerful tool for functional genomic studies. For many microarrays, pieces of DNA are deposited onto known locations on specially coated glass slides, and then these arrays are hybridized with fluorescently labeled cDNA molecules (1). One important aspect of all microarray studies is the replication of data; for the microarray community, sample replicates are of the utmost...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- BioTechniques
دوره 35 4 شماره
صفحات -
تاریخ انتشار 2003